!!NA_SEQUENCE 1.0

Plasmid pACYC177

Update 5/24/02

Features:

3699- 618 beta-lactamase (bla; amp-r) CDS (start 3699)

3699-3767 beta-lactamase signal peptide (start 3699)

783-1328 p15A origin of replication (clockwise)

(RNAII -35 to RNA/DNA switch point)

1923-2738 Tn903 kanamycin resistance CDS (start 1923)

pacyc177.seq Length: 3941 May 24, 2002 14:49 Type: N Check: 2062 ..

1 GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA

51 TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA

101 TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT

151 GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC

201 TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC

251 CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT

301 GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC

351 TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG

401 CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT

451 AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG

501 GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC

551 AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA

601 CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA

651 GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC

701 TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC

751 TGAGCGTCAG ACCCCTTAAT AAGATGATCT TCTTGAGATC GTTTTGGTCT

801 GCGCGTAATC TCTTGCTCTG AAAACGAAAA AACCGCCTTG CAGGGCGGTT

851 TTTCGAAGGT TCTCTGAGCT ACCAACTCTT TGAACCGAGG TAACTGGCTT

901 GGAGGAGCGC AGTCACCAAA ACTTGTCCTT TCAGTTTAGC CTTAACCGGC

951 GCATGACTTC AAGACTAACT CCTCTAAATC AATTACCAGT GGCTGCTGCC

1001 AGTGGTGCTT TTGCATGTCT TTCCGGGTTG GACTCAAGAC GATAGTTACC

1051 GGATAAGGCG CAGCGGTCGG ACTGAACGGG GGGTTCGTGC ATACAGTCCA

1101 GCTTGGAGCG AACTGCCTAC CCGGAACTGA GTGTCAGGCG TGGAATGAGA

1151 CAAACGCGGC CATAACAGCG GAATGACACC GGTAAACCGA AAGGCAGGAA

1201 CAGGAGAGCG CACGAGGGAG CCGCCAGGGG GAAACGCCTG GTATCTTTAT

1251 AGTCCTGTCG GGTTTCGCCA CCACTGATTT GAGCGTCAGA TTTCGTGATG

1301 CTTGTCAGGG GGGCGGAGCC TATGGAAAAA CGGCTTTGCC GCGGCCCTCT

1351 CACTTCCCTG TTAAGTATCT TCCTGGCATC TTCCAGGAAA TCTCCGCCCC

1401 GTTCGTAAGC CATTTCCGCT CGCCGCAGTC GAACGACCGA GCGTAGCGAG

1451 TCAGTGAGCG AGGAAGCGGA ATATATCCTG TATCACATAT TCTGCTGACG

1501 CACCGGTGCA GCCTTTTTTC TCCTGCCACA TGAAGCACTT CACTGACACC

1551 CTCATCAGTG CCAACATAGT AAGCCAGTAT ACACTCCGCT AGCGCTGAGG

1601 TCTGCCTCGT GAAGAAGGTG TTGCTGACTC ATACCAGGCC TGAATCGCCC

1651 CATCATCCAG CCAGAAAGTG AGGGAGCCAC GGTTGATGAG AGCTTTGTTG

1701 TAGGTGGACC AGTTGGTGAT TTTGAACTTT TGCTTTGCCA CGGAACGGTC

1751 TGCGTTGTCG GGAAGATGCG TGATCTGATC CTTCAACTCA GCAAAAGTTC

1801 GATTTATTCA ACAAAGCCAC GTTGTGTCTC AAAATCTCTG ATGTTACATT

1851 GCACAAGATA AAAATATATC ATCATGAACA ATAAAACTGT CTGCTTACAT

1901 AAACAGTAAT ACAAGGGGTG TTATGAGCCA TATTCAACGG GAAACGTCTT

1951 GCTCGAGGCC GCGATTAAAT TCCAACATGG ATGCTGATTT ATATGGGTAT

2001 AAATGGGCTC GCGATAATGT CGGGCAATCA GGTGCGACAA TCTATCGATT

2051 GTATGGGAAG CCCGATGCGC CAGAGTTGTT TCTGAAACAT GGCAAAGGTA

2101 GCGTTGCCAA TGATGTTACA GATGAGATGG TCAGACTAAA CTGGCTGACG

2151 GAATTTATGC CTCTTCCGAC CATCAAGCAT TTTATCCGTA CTCCTGATGA

2201 TGCATGGTTA CTCACCACTG CGATCCCCGG GAAAACAGCA TTCCAGGTAT

2251 TAGAAGAATA TCCTGATTCA GGTGAAAATA TTGTTGATGC GCTGGCAGTG

2301 TTCCTGCGCC GGTTGCATTC GATTCCTGTT TGTAATTGTC CTTTTAACAG

2351 CGATCGCGTA TTTCGTCTCG CTCAGGCGCA ATCACGAATG AATAACGGTT

2401 TGGTTGATGC GAGTGATTTT GATGACGAGC GTAATGGCTG GCCTGTTGAA

2451 CAAGTCTGGA AAGAAATGCA TAAGCTTTTG CCATTCTCAC CGGATTCAGT

2501 CGTCACTCAT GGTGATTTCT CACTTGATAA CCTTATTTTT GACGAGGGGA

2551 AATTAATAGG TTGTATTGAT GTTGGACGAG TCGGAATCGC AGACCGATAC

2601 CAGGATCTTG CCATCCTATG GAACTGCCTC GGTGAGTTTT CTCCTTCATT

2651 ACAGAAACGG CTTTTTCAAA AATATGGTAT TGATAATCCT GATATGAATA

2701 AATTGCAGTT TCATTTGATG CTCGATGAGT TTTTCTAATC AGAATTGGTT

2751 AATTGGTTGT AACACTGGCA GAGCATTACG CTGACTTGAC GGGACGGCGG

2801 CTTTGTTGAA TAAATCGAAC TTTTGCTGAG TTGAAGGATC AGATCACGCA

2851 TCTTCCCGAC AACGCAGACC GTTCCGTGGC AAAGCAAAAG TTCAAAATCA

2901 CCAACTGGTC CACCTACAAC AAAGCTCTCA TCAACCGTGG CTCCCTCACT

2951 TTCTGGCTGG ATGATGGGGC GATTCAGGCC TGGTATGAGT CAGCAACACC

3001 TTCTTCACGA GGCAGACCTC AGCGCTCAAA GATGCAGGGG TAAAAGCTAA

3051 CCGCATCTTT ACCGACAAGG CATCCGGCAG TTCAACAGAT CGGGAAGGGC

3101 TGGATTTGCT GAGGATGAAG GTGGAGGAAG GTGATGTCAT TCTGGTGAAG

3151 AAGCTCGACC GTCTTGGCCG CGACACCGCC GACATGATCC AACTGATAAA

3201 AGAGTTTGAT GCTCAGGGTG TAGCGGTTCG GTTTATTGAC GACGGGATCA

3251 GTACCGACGG TGATATGGGG CAAATGGTGG TCACCATCCT GTCGGCTGTG

3301 GCACAGGCTG AACGCCGGAG GATCCTAGAG CGCACGAATG AGGGCCGACA

3351 GGAAGCAAAG CTGAAAGGAA TCAAATTTGG CCGCAGGCGT ACCGTGGACA

3401 GGAACGTCGT GCTGACGCTT CATCAGAAGG GCACTGGTGC AACGGAAATT

3451 GCTCATCAGC TCAGTATTGC CCGCTCCACG GTTTATAAAA TTCTTGAAGA

3501 CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT

3551 AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA

3601 CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG

3651 AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT

3701 GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT

3751 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT

3801 GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG

3851 CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA

3901 GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG T