Ethanol Precipitation

 

  1. Add 10% 3M NaOAc pH 5.2
  2. Add 2.5X 95% EtOH
  3. Let sit at -80 C for 15min
  4. Spin at max speed @4 C for 15min
  5. Aspirate 95% EtOH
  6. Wash with 70% EtOH
  7. Let sit at -20 C for 5min
  8. Spin @ max speed @4 C for 8min
  9. Aspirate 70% EtOH

   10. Re-suspend in sterile water ~25 uL

   11. Store at -20 C