Ethanol Precipitation
- Add 10% 3M
NaOAc pH 5.2
- Add 2.5X 95%
EtOH
- Let sit at -80
C for 15min
- Spin at max
speed @4 C for 15min
- Aspirate 95%
EtOH
- Wash with 70%
EtOH
- Let sit at -20
C for 5min
- Spin @ max
speed @4 C for 8min
- Aspirate 70%
EtOH
10. Re-suspend in sterile water
~25 uL
11. Store at -20 C