Oligonucleotide purification via Sepak C18 columns

1. Re-suspend the oligo in 1mL dH2O, heat to 90C for 5 minutes and immediately cool on ice. Spind down for 1 minute at 4C.

2. Run the following across a Sepak C18 column using a 10 mL syringe and a SLOW flow rate:

a. 10mL 100% acetonitrile.

b. 10mL dH2O.

c. load oligo.

d. 10mL dH2O to wash.

e. 3mL 10% methanol.

f. 3mL 70% methanol.

3. Retain the 10% and 70% methanol elutions in separate tubes, small oligos should elute in the 10% fraction.

4. Oligos may now be diluted to the desired concentration and dried. Store at –20 C.