Gel Recipes
5 % Separating Gel
|
|
10mL |
20mL |
30mL |
40mL |
50mL |
|
H2O (mL) |
1.2 |
2.4 |
3.6 |
4.8 |
6.0 |
|
Acrylamide (30%) (mL) |
1.7 |
3.4 |
5.1 |
6.8 |
8.5 |
|
Gel Buffer A (mL) |
7.0 |
14.0 |
21.0 |
28.0 |
35.0 |
|
APS (10%) (uL) |
100.0 |
200.0 |
300.0 |
400.0 |
500.0 |
|
TEMED (uL) |
8.0 |
16.0 |
24.0 |
32.0 |
40.0 |
7.5 % Separating Gel
|
|
10mL |
20mL |
30mL |
40mL |
50mL |
|
H2O (mL) |
0.8 |
1.6 |
2.4 |
3.2 |
4.1 |
|
Acrylamide (30%) (mL) |
2.5 |
5.0 |
7.5 |
10.0 |
12.5 |
|
Gel Buffer A (mL) |
7.0 |
14.0 |
21.0 |
28.0 |
35.0 |
|
APS (10%) (uL) |
22.0 |
66.0 |
99.0 |
132.0 |
165.0 |
|
TEMED (uL) |
5.8 |
11.6 |
17.4 |
23.2 |
29.0 |
10 % Separating Gel
|
|
10mL |
20mL |
30mL |
40mL |
50mL |
|
H2O (mL) |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
|
Acrylamide (30%) (mL) |
3.3 |
6.7 |
10.0 |
13.3 |
16.7 |
|
Gel Buffer A (mL) |
7.0 |
14.0 |
21.0 |
28.0 |
35.0 |
|
APS (10%) (uL) |
33.0 |
66.0 |
99.0 |
132.0 |
165.0 |
|
TEMED (uL) |
3.3 |
6.6 |
9,9 |
13.2 |
16.5 |
Stacking Gel
|
|
10mL |
20mL |
30mL |
40mL |
50mL |
|
H2O (mL) |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
|
Acrylamide (30%) (mL) |
0.4 |
0.8 |
1.2 |
1.6 |
2.0 |
|
Stacking Buffer (mL) |
2.1 |
4.2 |
6.3 |
8.4 |
10.5 |
|
APS (10%) (uL) |
20.0 |
40.0 |
60.0 |
80.0 |
100.0 |
|
TEMED (uL) |
2.0 |
4.0 |
6.0 |
8.0 |
10.0 |