Transforming Cells

1. Mix 100uL of competent cells with 1uL of your mini-prep DNA
2. Use 100uL of competent cells alone as a negative control (No DNA added)
3. Incubate the tubes on an ice bath for 30 minutes
4. Heat shock the cells / DNA mixture at 42 degrees for 45 seconds
5. Incubate the tubes on an ice bath for 5 more minutes
6. Add 1mL of LB to each of the tubes
7. Incubate the stocks at 37 degrees for 45 minutes in the shaker
8. Plate out 20uL, 200uL, and if necessary an extra plate with 100uL of each sample onto the selective media
9. Incubate at 37 degrees overnight.